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RT-PCR Analyses of DSCAM expression
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a) DSCAM expression in human fetal heart and brain. RT-PCR
was performed using primers which flank the transmembrane domain of
DSCAM. C = control, H = heart, B = brain, M = molecular weight marker.
The reactions were templated on cDNAs encoding the deleted (1) and
full-length (2) DSCAM transcripts as controls, and on cDNAs reverse-transcribed
from human fetal heart (3) and brain (4) at 12 weeks of development,
from pooled human fetal heart samples at 19-23 weeks of development
(5) and from human fetal brain at 32 weeks of development
(6).
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b) DSCAM expression in mouse fetal heart. RT-PCR was performed
using two different primer pairs as described in the methods. The
reactions were templated on cDNA encoding mouse dscam (lanes 1 &
3) and a mouse
fetal heart cDNA library at E10.5 p.c. (lanes 2 & 4).
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