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Mouse Molecular Cytogenetic Resource:
157 BACs Link the Chromosomal and Genetic Maps:
Genome Res. 1999 May;9(5):514-23.
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Abstract:
Korenberg JR, Chen
XN, Devon KL, Noya D, Oster-Granite ML, Birren BW.
Medical Genetics Birth Defects Center, Cedars-Sinai Medical Center,
University of California, Los Angeles, Los Angeles, California 90048,
USA.
We have established a collection of strong molecular cytogenetic markers
that span the mouse autosomes and X chromosome at an average spacing of
one per 19 Mb and identify 127 distinct band landmarks. In addition, this
Mouse Molecular Cytogenetic Resource relates the ends of the genetic maps
to their chromosomal locations. The resource consists of 157 bacterial
artificial chromosome (BAC) clones, each of which identifies specific
mouse chromosome bands or band borders, and 42 of which are linked to
genetic markers that define the centromeric and telomeric ends of the
Whitehead/MIT recombinational maps. In addition, 108 randomly selected
and 6 STS-linked BACs have been assigned to single chromosome bands. We
have also developed a high-resolution fluorescent reverse-banding technique
for mouse chromosomes that allows simultaneous localization of probes
by fluorescence in situ hybridization (FISH) with respect to the cytogenetic
landmarks. This approach integrates studies of the entire mouse genome.
Moreover, these reagents will simplify gene mapping and analyses of genomic
fragments in fetal and adult mouse models. As shown with the MMU16 telomeric
marker for the trisomy 16 mouse model of Down syndrome, these clones can
obviate the need for metaphase analyses. The potential contribution of
this resource and associated methods extends well beyond mapping and includes
clues to understanding mouse chromosomes and their rearrangements in cancers
and evolution. Finally it will facilitate the development of an integrated
view of the mouse genome by providing anchor points from the genetic to
the cytogenetic and functional maps of the mouse as we attempt to understand
mutations, their biological consequences, and gene function.
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