Vector Development
  • Smith-Arica, J., Morelli, A.E., Larregina, A., Smith, J., Lowenstein, P.R. and Castro, M.G. Cell-type-specific and regulatable transgenesis in the adult brain: adenovirus-encoded combined transcriptional targeting and inducible transgene expression. Molecular Therapy, 2: 579-587, 2000. Click here for the PDF

  • Brown, O., Cowen, R.L., Preston, C.M., Castro, M.G. and Lowenstein. P.R. Non-polarized neuronal targeting of a glycosylphosphatidylinositol (GPI) anchored protein: viral vector delivery of an exogenous GPI-anchored reporter protein into polarized post mitotic neocortical or hippocampal neurons in vitro results in its localization to both the somato-dendritic and axonal plasma membrane domains. Gene Therapy, 7: 1947-1953, 2000. Click here for the PDF

  • Umana, P., C. A. Gerdes, D. Stone, J. R. Davis, D. Ward, M. G. Castro and P. R. Lowenstein (2001). "Efficient FLPe recombinase enables scalable production of helper-dependent adenoviral vectors with negligible helper-virus contamination." Nat Biotechnol 19(6): 582-5. Click here for the PDF

  • Lowenstein P.R., Thomas C.E., Umana P, Gerdes C.A., Verakis T., Boyer O., Tondeur S., Klatzmann D, Castro M.G. High-capacity, helper-dependent, "gutless" adenoviral vectors for gene transfer into brain. Methods Enzymol. 2002; 346:292-311. Click here for the PDF

  • Brown, O., et al. (2000). "Subcellular post-transcriptional targeting: delivery of an intracellular protein to the extracellular leaflet of the plasma membrane using a glycosyl-phosphatidylinositol (GPI) membrane anchor in neurons and polarised epithelial cells." Gene Ther 7(22): 1947-53. Click here for the PDF

  • Cowen, R. L., J. C. Williams, S. Emery, D. Blakey, J. L. Darling, P. R. Lowenstein and M. G. Castro (2002). "Adenovirus vector-mediated delivery of the prodrug-converting enzyme carboxypeptidase G2 in a secreted or GPI-anchored form: High-level expression of this active conditional cytotoxic enzyme at the plasma membrane." Cancer Gene Ther 9(11): 897-907. Click here for the PDF

  • Larregina, A. T., A. E. Morelli, R. A. Dewey, M. G. Castro, A. Fontana and P. R. Lowenstein (1998). "FasL induces Fas/Apo1-mediated apoptosis in human embryonic kidney 293 cells routinely used to generate E1-deleted adenoviral vectors." Gene Ther 5(4): 563-8.

  • Lowenstein, P. R., C. E. Thomas, P. Umana, C. A. Gerdes, T. Verakis, O. Boyer, S. Tondeur, D. Klatzmann and M. G. Castro (2002). "High-capacity, helper-dependent, "gutless" adenoviral vectors for gene transfer into brain." Methods Enzymol 346: 292-311. Click here for the PDF

  • Southgate, T., Kingston, P., and Castro, M.G. (2000). Gene transer into neural cells in vivo using adenoviral vectors. Current Protocols in Neuroscience. C. R. Gerfen, McKay, R., Rogawski, M.A., Sibley, D.R., Skolnick, P. New York, NY, John Wiley and Sons, New York. 4.23.1-4.23.40: 4.23.1-4.23.40. Click here for the PDF
  • Thomas CE, Schiedner G, Kochanek S, Castro MG, Lowenstein PR."Preexisting antiadenoviral immunity is not a barrier to efficient and stable transduction of the brain, mediated by novel high-capacity adenovirus vectors."Hum Gene Ther. 2001 May 1;12(7):839-46. Click here for the PDF

  • Thomas CE, Edwards P, Wickham TJ, Castro MG, Lowenstein PR."Adenovirus binding to the coxsackievirus and adenovirus receptor or integrins is not required to elicit brain inflammation but is necessary to transduce specific neural cell types." J Virol. 2002 Apr;76(7):3452-60. Click here for the PDF

  • Appleby CE, Kingston PA, David A, Gerdes CA, Umana P, Castro MG, Lowenstein PR, Heagerty AM."Real-time imaging of gene promoter activity using an adenoviral reporter construct demonstrates transcriptional dynamics in normal anterior pituitary cells." J Endocrinol. 2003 Jul;178(1):61-9.

  • Lowenstein PR, Suwelack D, Hu J, Yuan X, Jimenez-Dalmaroni M, Goverdhana S, Castro MG."Nonneurotropic adenovirus: a vector for gene transfer to the brain and gene therapy of neurological disorders."Int Rev Neurobiol. 2003;55:3-64 Click here for the PDF

  • Dorigo O, Gil JS, Gallaher SD, Tan BT, Castro MG, Lowenstein PR, Calos MP, Berk AJ."Development of a novel helper-dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cells." J Virol. 2004 Jun;78(12):6556-66. Click here for the PDF

  • Goverdhana S, Puntel M, Xiong W, Zirger JM, Barcia C, Curtin JF, Soffer EB, Mondkar S, King GD, Hu J, Sciascia SA, Candolfi M, Greengold DS, Lowenstein PR, Castro MG."Regulatable gene expression systems for gene therapy applications: progress and future challenges." Mol Ther. 2005 Aug;12(2):189-211 Click here for the PDF

  • Abordo-Adesida E, Follenzi A, Barcia C, Sciascia S, Castro MG, Naldini L, Lowenstein PR."Stability of lentiviral vector-mediated transgene expression in the brain in the presence of systemic antivector immune responses." Hum Gene Ther. 2005 Jun;16(6):741-51. Click here for the PDF

  • Xiong W, Goverdhana S, Sciascia SA, Candolfi M, Zirger JM, Barcia C, Curtin JF, King GD, Jaita G, Liu C, Kroeger K, Agadjanian H, Medina-Kauwe L, Palmer D, Ng P, Lowenstein PR, Castro MG."Regulatable gutless adenovirus vectors sustain inducible transgene expression in the brain in the presence of an immune response against adenoviruses." J Virol. 2006 Jan;80(1):27-37. Click here for the PDF

  • Puntel M., Zirger J, Xiong W, Hu J, Yuan X, Liu C, Curtin J, Kroeger K, Czer P, Williamson IK, Lowenstein PR, Castro MG, (2005) Molecular titration of high-capacity helper dependent adenoviral vectors. Human Gene Therapy 17(5):531-44. Click here for the PDF

  • Candolfi M, Kroeger KM, Pluhar GE, Bergeron J, Puntel M, Curtin JF, McNiel EA, Freese AB, Ohlfest JR, Moore P, Lowenstein PR, Castro MG. Adenoviral-mediated gene transfer into the canine brain in vivo. Neurosurgery. 2007 Jan;60(1):167-77 Click here for the PDF

  • Candolfi M, Pluhar GE, Kroeger K, Puntel M, Curtin J, Barcia C, Muhammad AK, Xiong W, Liu C, Mondkar S, Kuoy W, Kang T, McNeil EA, Freese AB, Ohlfest JR, Moore P, Palmer D, Ng P, Young JD, Lowenstein PR, Castro MG. Optimization of adenoviral vector-mediated transgene expression in the canine brain in vivo, and in canine glioma cells in vitro. Neuro Oncol. 2007 Jul;9(3):245-58 Click here for the PDF

  • Zirger, J.M., Barcia, C., Liu, C., Puntel, M., Michell, N., Campbell, I., Castro, MG., Lowenstein, P.R. (2006) Rapid upregulation of IFN-regulated and chemokine-mRNAs upon injection of 1 x 108 iu, but not lower doses of adenoviral vectors into the brain. The Journal of Virology 80:5644-5659 Click here for the PDF

  • Zirger JM, Liu C, Barcia C, Castro MG, Lowenstein PR (2006) Immune regulation of transgene expression in the brain: B cells regulate an early phase of elimination of transgene expression from adenoviral vectors. Viral Immunology, 19(3):508-17. Click here for the PDF

  • Candolfi Marianela , G. Elizabeth Pluhar, Kurt Kroeger1, Mariana Puntel1, James Curtin, Carlos Barcia, AKM Ghulam Muhammad, Weidong Xiong, Chunyan Liu, Sonali Mondkar, William Kuoy, Terry Kang, Elizabeth A. McNeil, Andrew B. Freese,John R. Ohlfest, Peter Moore, Donna Palmer, Phillip Ng, John D. Young, Pedro R. Lowenstein1, Maria G. Castro. "Optimization of adenoviral vector mediated expression in the canine brain in vivo, and in canine glioma cells in vitro." Neuro Oncol. 2007 Jul;9(3):245-58 Click here for the PDF

  • Oh S, Pluhar GE, McNeil EA, Kroeger KM, Liu C, Castro MG, Lowenstein PR, Freese A, Ohlfest JR. Efficacy of nonviral gene transfer in the canine brain. J Neurosurg. 2007 Jul;107(1):136-44. Click here for the PDF

  • Oh S, Odland R, Wilson SR, Kroeger KM, Liu C, Lowenstein PR, Castro MG, Hall WA, Ohlfest JR. Improved distribution of small molecules and viral vectors in the murine brain using a hollow fiber catheter. J Neurosurg. 2007 Sep;107(3):568-77.

  • Barcia C, Jimenez-Dalmaroni M, Kroeger KM, Puntel M, Rapaport AJ, Larocque D, King GD, Johnson SA, Liu C, Xiong W, Candolfi M, Mondkar S, Ng P, Palmer D, Castro MG, Lowenstein PR. One-year Expression From High-capacity Adenoviral Vectors in the Brains of Animals With Pre-existing Anti-adenoviral Immunity: Clinical Implications. Mol Ther. 2007 Dec;15(12):2154-2163 Click here for the PDF

  • Lowenstein PR, Kroeger K, Castro MG. Immunology of neurological gene therapy: how T cells modulate viral vector-mediated therapeutic transgene expression through immunological synapses. Neurotherapeutics. 2007 Oct;4(4):715-24. Review Click here for the PDF

  • Lowenstein PR, Mandel RJ, Xiong WD, Kroeger K, Castro MG. Immune Responses to Adenovirus and Adeno-Associated Vectors Used for Gene Therapy of Brain Diseases: The Role of Immunological Synapses in Understanding the Cell Biology of Neuroimmune Interactions. Curr Gene Ther. 2007 Oct;7(5):347-360. Click here for the PDF

  • Barcia C, Gerdes C, Xiong WD, Thomas CE, Liu C, Kroeger KM, Castro MG, Lowenstein PR. Immunological thresholds in neurological gene therapy: highly efficient elimination of transduced cells might be related to the specific formation of immunological synapses between T cells and virus-infected brain cells. Neuron Glia Biol. 2007 Jul 13;(2):309-322. Click here for the PDF

  • Xiong W, Candolfi M, Kroeger KM, Puntel M, Mondkar S, Larocque D, Liu C, Curtin JF, Palmer D, Ng P, Lowenstein PR, Castro MG. Immunization Against the Transgene but not the TetON Switch Reduces Expression From Gutless Adenoviral Vectors in the Brain. Mol Ther. 2008 Feb;16(2):343-351. Click here for the PDF